Gardner lab home

Gardner lab protocols:

Please note that although the following protocols have worked well in our group, they are provided here on an as-is/use at your own risk basis.  Please contact us for more information!

uniform 15N, 15N/13C labeling in E. coli grown in M9 media

competent cell preparation

E. coli transformations

Terrific Broth preps

TEV protease preparation

thermal shift - ABI7500


Gardner lab wiki (local only)


Kevin Gardner <Kevin.Gardner@utsouthwestern.edu>revised:  July 18, 2008